ABSTRACT
Objective::Ultra performance liquid chromatography (UPLC) fingerprint was used to evaluate the storage effect of different packaging and storage conditions on Notopterygh Rhizoma et Radix pieces. Method::UPLC fingerprints of Notopterygh Rhizoma et Radix pieces with different storage time and packaging conditions were established under following chromatographic conditions: Waters UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm), mobile phase 0.3%phosphoric acid solution(A)-acetonitrile (B) for gradient elution (0-4 min, 90%-47%A; 4-8 min, 47%A, ; 8-12 min, 47%-20%A; 12-16 min, 20%A; 16-18 min, 20%-90%A), flow rate of 0.2 mL·min-1, column temperature at 35 ℃, detection wavelength of 246 nm, and injection volume of 1.0 μL. The fingerprints were evaluated in terms of similarity, cluster analysis and principal component analysis. Result::The similarity evaluation showed that the UPLC fingerprint patterns of Notopterygh Rhizoma et Radix pieces with different storage time and different packaging and storage treatments were basically the same, and the similarity was above 0.95.Systematic clustering shows that Notopterygh Rhizoma et Radix pieces packaged in plastic bags, stored under light and shade and the initial live-action pieces were clustered into one group. Principal component analysis showed that Notopterygh Rhizoma et Radix pieces packaged in plastic bags, stored under light and shade had the highest comprehensive scores. Conclusion::Storage time, packaging material and storage temperature will not cause the increase or decrease of internal components of Notopterygh Rhizoma et Radix, but only affect the content of specific components. Plastic bag packaging, light and cool storage conditions are more suitable for the preservation of non-volatile oil components of Notopterygh Rhizoma et Radix.
ABSTRACT
Objective::To study the effect of different packaging methods and storage conditions on the quality of Notopterygii Rhizoma et Radix pieces, in order to determine the optimal packaging method and suitable storage conditions for Notopterygii Rhizoma et Radix pieces. Method::Different packaged Notopterygii Rhizoma et Radix pieces were stored in different environments in a one-year long-term stability experiment. The appearance, water content, extract content and volatile oil content of Notopterygii Rhizoma et Radix pieces were regularly observed. Result::During the 1-year storage period, the Notopterygii Rhizoma et Radix pieces under different packaging and storage conditions showed different degrees of quality changes. Among them, the samples packed in polyethylene plastic bags and polyethylene aluminum foil composite bags were better preserved. The fluctuations in water content of the sample packed in polyethylene plastic bags were relatively low, and the RSD value of water content during the month was less than 11.5%. The extracts and volatile oil contents of each sample were reduced to different degree, but the samples packed in plastic sealed bags and protected from light had the smallest annual loss of extracts (1.27%), with the lowest monthly loss rate of volatile oil (0.08%). Conclusion::The quality of Notopterygii Rhizoma et Radix pieces can be well preserved in plastic sealed bags and storage in dark and cool conditions, which is suitable for the storage of Notopterygii Rhizoma et Radix pieces.
ABSTRACT
Objective To investigate the therapeutic effects of penetrating resin and fluoride on early enamel caries. Methods Sixty intact bovine incisors were immersed in demineralized solution for 24 hours to make bovine incisor enamel caries.The specimens were divided into four groups(n=15 for each group)according to the treatment methods:control group (CON)-immersion in artificial saliva, DF group-immersion in 0.05% fluoride solution daily, WF group-2% fluoride gel weekly and IC group-resin infiltration.After processing for four and eighe weeks,the microhardness of the surface of each group was measured.After the treatment for four weeks,the depth of penetration and the microhardness of the samples were measured. After 8-week treatment, all samples were reintroduced into the demineralized solution for 24 hours and the microhardness of the samples was measured again.Results Results of microhardness assessment showed that there were no significant differences in baseline values(after white spots)between four groups(P>0.05).After treatment for four weeks the microhardness value reached the peak in IC group. After treatment for eight weeks the microhardness values reached the peak in DF group and WF group.The values of microhardness were significantly higher at different time points in IC group than those of other groups(P<0.05).After 4-week treatment,the percentages of penetration depth were significantly higher in DF,WF and IC groups than those of control group(P<0.01).The penetration depth was significantly higher in IC group than that of DF group and WF group (P<0.05). There was no significant difference in the penetration depth between DF group and WF group(P>0.05).Conclusion For the early enamel caries penetration resin,the penetration percentage is significantly higher than the fluoride treatment.